Immunoassay Testing
On-site, 60-minute Results!
Calibrated to Idexx Reference Lab/Immulite analyzer
Immunoassay testing is an investigative procedure to quantitatively assess and measure the presence and/or amount or functional activity of a target analyte. The analyte can be progesterone, thyroid, etc.
RED HILLS REPRO* offers 60-minute, on-site Progesterone testing which requires 1 cc of blood to be spun down in a centrifuge and the blood serum analyzed. A written printout will be provided at time of service for these results and how to interpret them.
Our analyzer is calibrated to the Idexx Reference Lab Immulite, which is one we are most familiar with when we have our labs sent out for overnight results. We have found the results from our analyzer are very accurate without the wait time, improving care by delivering results in minutes, It eliminate 'estimates' and ambiguity and the need for reference lab confirmation. With 60-minute results, our testing allows for faster initiation of treatment or the initiation of time sensitive breeding plans...all that can be further discussed and planned with your veterinarian.
Our progesterone assay is priced at $99 and is done on-site in our mobile lab with 60 minute results! You can wait for results or we can text them to you while at the show.
PROGESTERONE
Progesterone is a hormone produced by the ovaries and placenta that helps prepare and maintain the uterus for pregnancy. Our canine Progesterone is a quantitative measurement of progesterone concentration in canine serum. Progesterone levels begin to rise around the time of LH (Luteinizing Hormone) surge. Thus, the production starts prior to ovulation at the end of proestrus and reaches increased levels at the time of ovulation.
Our canine Progesterone test allows for the identification of optimal breeding dates and reproductive disorders, allowing veterinarians to quickly prescribe decisive breeding plans, to predict parturition dates or time a Cesarean section, and to detect reproductive disorders such as split heats, delayed puberty, silent estrus or hypoluteidism. The provide quantitative data that can be further utilized by your veterinarian.
*Red Hills Repro does not offer veterinary advise, but rather provides data and immunoassay results that should always be followed up by your veterinarian to diagnose, prescribe and treat your canine with the best possible course of action.
BRUCELLOSIS
Canine brucellosis is a contagious bacterial infection caused by the bacterium, Brucella canis (B. canis). This bacterial infection is highly contagious between dogs. Infected dogs usually develop an infection of the reproductive system, or a sexually transmitted disease.
Different species of Brucella infect sheep, goats, cattle, deer, elk, pigs, humans and other animals.
What are the signs of brucellosis?
Brucellosis in dogs typically causes reproductive problems such as infertility and abortions, with few other signs of clinical illness. The disease is most common in sexually intact adult dogs.
Male dogs infected with brucellosis develop epididymitis, an infection in part of the testicle. A dog with a newly acquired infection will often have an enlarged scrotum or an enlarged testicle and may have a skin rash on the scrotum. The dog may be infertile. In chronic or long-standing cases, the testicles will atrophy or become shrunken.
Female dogs infected with brucellosis develop an infection of the uterus; causing her to be infertile, have difficulty getting pregnant, or she may abort in the late stages of pregnancy. She often has a persistent vaginal discharge. Typically, a pregnant dog with brucellosis will abort at 45-55 days of gestation or will give birth to stillborn or weak puppies that may die a few days after birth.
During the early stages of brucellosis, enlarged lymph nodes are common. Occasionally, B. canis will infect the intervertebral discs, eyes, kidneys, or brain. If the bacteria infects these other tissues, the signs will be related to the bodily system that is infected.
How is canine brucellosis spread?
Large numbers of B. canis bacteria are shed in the genital secretions (semen or vaginal discharges) of an infected dog. Smaller amounts of bacteria may also be shed in the dog's urine or saliva. After a female dog aborts a pregnancy because of brucellosis, she will continue to discharge fluids infected with the bacteria for 4-6 weeks after the abortion.
Dogs are exposed to the disease via contact with infected bodily fluids. Although the most common route of infection is oral (i.e., from licking contaminated urine or discharges from the reproductive tract or licking or chewing placental material or aborted fetuses), dogs can also pick up an infection through sexual transmission, inhalation (sniffing contaminated urine or other discharges), or through other mucous membranes such as the eyes.
How is canine brucellosis diagnosed?
The infection is usually diagnosed by a blood test. RED HILLS offers 30-minute brucellosis testing on-site. The most common blood test is called a rapid slide agglutination test or RAST, and it can detect infections after three to four weeks. This test is used for screening of breeding dogs, and negative tests are reliable unless the dog has been recently exposed to the disease. False-positive tests are relatively common, and any dog that tests positive with the RAST test should have the disease confirmed with an advanced test called an agar gel immunodiffusion test (AGID), which will identify infected animals between 12 weeks and 1 year post-infection. This AGID test can only be performed by a licensed veterinarian.
What is the treatment for canine brucellosis?
Although antibiotics (most often minocycline or doxycycline, possibly enrofloxacin) can be used to help control the infection, no treatment is completely effective at eliminating the bacteria, and any dog that has been infected with B. canis should be considered to be infected for life. Even if the acute infection can be controlled with antibiotics, the dog may shed bacteria intermittently for the rest of its life.
Surgical sterilization of the infected dog will decrease shedding of the organisms into the environment, thereby reducing the risk to other dogs.
How can brucellosis be controlled?
Brucellosis in dogs is a rare disease. It is more prevalent in some areas of the United States, such as the southern USA, and in other parts of the world. Since the disease is a major threat to the breeding capability of dogs, all dogs used for breeding purposes should be tested regularly (e.g., every 3-6 months, depending on exposure to other dogs), and new dogs should never be introduced into a kennel situation until they have been quarantined for 8-12 weeks and then tested for the disease. Most experts recommend performing two blood tests four weeks apart, near the end of the quarantine period.
In the United States, brucellosis is a reportable disease, meaning that the disease is of great public health importance, and veterinarians and physicians are required to report all positive cases to federal authorities. Canine brucellosis is not reportable in Canada.
Am I at risk for developing brucellosis from an infected dog?
Brucellosis is a zoonotic disease, or a disease that can be transmitted from animals to humans. Although people can become infected by coming in contact with infected animals, it is uncommon for a person to get a brucellosis infection from a dog.
Breeders and veterinarians exposed to the blood or other secretions of infected animals are at an increased risk of developing an infection; pet owners are not considered to be at risk for infection because they are less likely to come in contact with blood, semen, or uterine discharges from an infected dog.
However, people with compromised immune systems should avoid contact with a dog that is diagnosed with brucellosis.
People who come in contact with breeding dogs, newborn puppies, or aborted fetuses should use caution and practice good sanitation. Whenever possible, wear disposable gloves before handling newborn puppies or cleaning an area where a dog has whelped. After removal of the disposable gloves, wash your hands thoroughly with soap and water and rinse well.
(RED HILLS would like to emphasize the importance of semen collection facilities to wear gloves at all times when handling dogs because this bacteria can be spread to healthy dogs while being collected if precautions are not taken to minimize exposure. Demand that your collectors glove up before touching your dogs!)
Contributors: Ryan Llera, BSc, DVM; Cheryl Yuill, DVM, MSc, CVH
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